Composite
Weak TP

Part:BBa_K2918017:Design

Designed by: TUDelft2019   Group: iGEM19_TUDelft   (2019-10-01)


Weak T7 promoter - Universal RBS - Φ29 TP - WT T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MoClo overhangs


Source

MoClo Assembly

References